(i) What should the pH of your buffer be if you wanted to bind HMD to an anionexchange column? Why?
(ii) What should the pH of your buffer be if you want to bind HMD to a cation-exchange column? Why?
(iii) What potential issues might arise if the pH of your buffer is too close to the pI of your protein of interest? Why might an extreme buffer pH (< 6 or > 9) be unsuitable?
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