Determine the maximum flow rate for the centrifugation of a suspension of lysed bacteria cells by a plant-scale disk centrifuge.
Disk/bowl centrifuge characteristics (see Attachment)
Bowl diameter = 25.4 cm
Angle (Φ’) = 42°
Distance to inside edge of disk (r1) = 8 cm
Distance to outside edge of disk (r2) = 20 cm
Number of disks (n’) = 100
In a laboratory centrifuge, it was determined that it took 17 minutes to clarify the lysed cells at 12,000 rpm. The laboratory centrifuge the top of the liquid was 32 mm and the packed solid was 79 mm from the center of rotation, respectively.