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CK-2_Beer's_Law_and_Colorimetry_RPT.pdf

Lab Report Assistant: This document is not meant to be a substitute for a formal
laboratory report. It is simply a summary of the experiments questions and data tables
which should be addressed in a formal lab report. The intent is to facilitate students
writing of lab reports by providing this information in an .rtf format which can be copied
and pasted into an electronic document.

Beers Law & Colorimetry
Part 1: Beer's Law:

1. Prior to analyzing the samples provided by Q you must demonstrate (for yourself
and others) that you, your instruments, and your company will produce reliable
results. One of the ways you decide to do this is by making a Beers law plot using
pure M. You obtain the following data:
Sample Identification
Code
Q5000

Concentration of M
(mol/L)
4.00 x 10-4

%T
17.9

Q5001

3.20 x 10-4

25.0

Q5002

2.40 x 10-4

35.7

Q5003

1.60 x 10-4

50.2

Q5004

8.000 x 10-5

A

70.8

Convert %T to absorbance and prepare a Beers law plot using these data.
2. You are now ready to begin analyzing the drug. You are sent five bottles of the drug
from batch 021015. You analyze these samples and obtain the following data:
Sample Identification Code

%T

Q021015-01

43.7

Q021015-02

44.1

Q021015-03

43.8

Q021015-04

44.1

Q021015-05

43.8

What is the concentration of M in these samples?

A

3. Company Q has reported that batch 021015 has an M concentration of 3.00 x 10 -4
mol/L. Your analysis is better than theirs; therefore, you assume that your analysis is
correct. What was their percent error? Remember from Chemistry 1, that formula is:
% error = theoretical value actual value x 100%
theoretical value
4

By law, company Q must have an M concentration of 3.00 x 10 -4 mol/L +/- 5%. (In
other words, the M concentration must be between 2.85 x 10 -4 mol/L and 3.15 x 10-4
mol/L.) Does Batch 021015 meet legal requirements?

Part 2: Colorimetry of FD & C Blue # 1 Dye (Brilliant Blue FCF)
Data Table:
Well #

1

2

3

4

5

6

7

8

9

Drops of Blue Dye Solution

1

2

3

4

5

6

7

8

9

Drops of Distilled Water

9

8

7

6

5

4

3

2

1

Calculated Concentration

2

10
10
0

Lab Report Assistant: This document is not meant to be a substitute for a formal
laboratory report. It is simply a summary of the experiments questions and data tables
which should be addressed in a formal lab report. The intent is to facilitate students
writing of lab reports by providing this information in an .rtf format which can be copied
and pasted into an electronic document.
Part 3:
The student will use a colorimeter to analyze the concentration of a blue dye for a
quantitative analysis.
IMPORTANT NOTE You will only be completing Exercise 3 if you instructor
selected the colorimeter assembly, pictured below, to be included as a separate
option with your LabPaq. If you do not have the Colorimeter assembly skip this
exercise.
Data Table 2- Dilution Data Table:
Test
tube
Number
3
2
1
0

Solutions
Starting dilution (100 mL water and drops of blue#1
dye)
1:1 Dilution (1 part water - 1 part starting dye
solution)
2:1 Dilution (2 parts water - 1 part starting dye
solution)

Concentration Concentratio
(M)
n
(ppm)

0

Zero standard distilled water

0

Use of colorimeter & determination of concentrations:
Data Table 3 - Resistance vs. Concentration
Zero
standard
Concentration (M)
Concentration (ppm)
Resistance,
Resistance,
Average Resistance,

0

2:1
standard

1:1
standard

Original
dilution

Commercial
drink sample

Questions:
Fully discuss answers to the following questions. Yes or No responses are not
sufficient.
A. Was your calibration curve as linear as you expected?
B. Did you experience any drift of the resistance readings?
C. What is the equation of your best-fit line?
D. What commercial drink did you analyze?
E. Assuming that the same commercial drink was analyzed in both colorimetry
methods, how did the results compare?
F. Which technique should give more accurate results?
G. What are some of the potential sources for errors in this experiment?

4

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