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At 37 °C the equilibrium concentration of Ca2+ inside a cell is 0.085 M and outside is 0.042 M. The cell membrane

is permeable to Ca2+. The only ion present in addition to Ca2+ and Cl– is a protein (Pr +/–) inside the cell. The protein holds a single positive or negative charge. The Cl– is at equilibrium across the membrane while the protein is inside the cell and cannot permeate the membrane. What is the concentration of Pr+/– (in M)?

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