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___________________ ID#:___________________ Chem 135 Spring 2015 Problem Set 3 Due in class, Thursday, February 12, 2015 1. Uridine vanadate is a...

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Name:___________________ ID#:___________________ Chem 135 Spring 2015 Problem Set 3 Due in class, Thursday, February 12, 2015 1. Uridine vanadate is a transition state (TS) analog inhibitor of ribonuclease A (RNase A), the mechanism of which we have seen in class. O HO N NH O O O O V HO HO O a) It is possible to obtain a crystal structure of RNase A bound to uridine vanadate, but not of RNase A bound to unmodified RNA. Briefly explain why (2-3 sentences). b) What type of inhibition do you expect uridine vanadate to display? (e.g. non-competitive, irreversible, etc.) Explain (2-3 sentences). c) Referring to the mechanism for RNase A, briefly explain why the structure of uridine vanadate makes it an effective inhibitor (2-3 sentences).
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Name:___________________ ID#:___________________ 2. Although rare, some proteins display negative cooperativity . (a) For a protein displaying negative cooperativity, how does K d vary as a function of substrate concentration? (b) Sketch the Hill plot for a protein displaying negative cooperativity. (c) For a protein with x subunits, what are the possible ranges of the Hill coefficient n if it displays i) positive cooperativity ii) no cooperativity iii) negative cooperativity.
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Ans 1) a) Uridine vanadate binds to RNase A in the anti conformation. The hydrogen bonds exist between the 2­carbonyl and 3­nitrogen position of its pyrimidine rings and complementary polar...

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