Hi I am trying to work out what I need to put in the pro forma for this experiment.
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Hi


I am trying to work out what I need to put in the pro forma for this experiment. I have

attached the experiment procedure and the results from the HPLC, along with the Proforma. We were given an excel spreadsheet to calculate the assess the calibration data and I am so confused what I need to do -


Q1a and b

Do I give one labelled diagram or all? Labelling the peaks of the one diagram?


Q1d

How do I calculate the UNCERTAINTY - Is it "The mass fraction of phenol in the effluent sample was 50.00 ± 0.35 mg/L, (where ± 0.35 mg/L represents a coverage interval that includes the value of the measurand with at least 95% probability)."


Q2a

Phenol can produce a toxic effect on humans through three key mechanisms of exposure:

1.    breathing air contaminated by phenol

2.    absorption through the skin or;

3.    by ingesting products contaminated with Phenol. (National Pollutant inventory database)

According to the Australian Water Quality Guidelines for Fresh and Marine Waters (ANZECC, 1992):

Maximum of 50 micrograms per Litre (i.e. 0.00005 g/L) in fresh or marine waters.


The oral LD50 value for humans has been reported as 140 mg/kg (Wiley, V-C-H, p.517)


Q3a

The concentrations of phenol and 2-cholorphenol that were detected in the industrial effluent do meet the guidelines for drinking water???

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