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Molecular Diagnostics Questions

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Exercise 1
1. Please match the following:
A. Replication
a. RNA dependent RNA synthesis
B. Transcription
b. RNA dependent protein synthesis
C. Translation
c. RNA dependent DNA synthesis
D. Reverse transcription
d. DNA dependent RNA synthesis
2. Given the following DNA sequence, what is the complementary strand DNA
sequence?
TCCGAACG
3. The following is a DNA coding stand sequence. What is the corresponding
RNA sequence?
TCCGAGGTA
4. What kind of nitrogenous base are they?
6
5
2
4
N9
H

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Exercise 2
1. Which of the following might be recognized by restriction enzyme?
ATGGTA
TACTAC
GCTAGC
AAAAAA
TGTGTG
2. A segment of was found to have the base sequence 5' AUCCCUGA. What
would be the DNA coding sequence from which it was transcribed?
3. List the enzyme players in DNA replication pathway in order. Make one
list for prokaryotes and one for eukaryotes.
4. List the names of disease/disorders and the enzymes involved in purine
metabolism errors.

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Exercise 3
1. What are the functions of Vitamin B12 and folate in thymidine
synthesis?
2. List the inhibitors of thymidine synthesis and tell me how its
associated in cancer chemotherapies.
3. What are the following structure?
DNA double
helix
DNA double
helix
4. Explain the mechanisms of DNA inhibitors Methotrexate, AZT, and
Aminopterin

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Exercise 4
1. In Eukaryotic organisms, what enzyme catalyze the synthesis of new
strand of DNA indicated by section B?
D
5'
A
C
E
2. Compare and contrast the enzymes involved in prokaryotic cell
transcription
3. Compare and contrast the enzymes involved in eukaryotic cell
transcription
4. What are these chemicals do during RNA transcription
A. Rifanpin
B. Actinomycin D
C. Dideoxyinosine

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