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WEEK 5: Page 8
Compiled Class Data Set
Transcribe the compiled class data from the excel spread sheet into Tables 5-3, 5-4, 5-5 and 5-6 after all crosses
have been scored. Use the class results, which should include your efforts, to complete the statistical analysis for
this project.
Table 5-3: Summary of wing and eye phenotypes in
Table 5-5: Summary of wing and eye phenotypes in
F1 generation derived from cross A.
F1 generation derived from cross B.
Cross A F1 Generation
Cross B F1 Generation
Wing
Eye
Females Males
Total
KO
Wing
Eye
Females
Males Total
Morph
Colour
Morph
Colour
Of
Normal
Red
312
312
Normal
Red
375
367 742
Vestigial
Red
Vestigial
Red
Normal
White
298
298
Normal
White
Vestigial
White
Vestigial
White
Table 5-4: Summary of wing and eye phenotypes in
Table 5-6: Summary of wing and eye phenotypes in
F2 generation derived from cross A.
F2 generation derived from cross B
Cross A F2 Generation
Cross B F2 Generation
Wing
Eye
Females Males
Total
Wing
Eye
Females
Males
Total
Morph
Colour
Morph
Colour
Normal
Red
18
198
Normal
Red
197
is
292
Vestigial
Red
Vestigial
Red
Normal
White
14
Normal
White
Vestigial
White
29
2
Vestigial
White
32
4 marks

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WEEK 5: Page 13
13. Throughout this analysis the phenotypes for two different genes (w and vg) were independently assessed.
Use proper nomenclature for both genes (w and vg), specify the genotypes of the following individuals. See
Supplemental document on transmission genetics for proper nomenclature conventions. 4 marks
a. P1 generation + flies used in cross A
b. P1 generation & flies used in cross A
c. F1 generation Is from cross A
d. F1 generation d's from cross A
14. Complete the Punnett Square below for Cross A. 10 marks
F10 gametes
F19 gametes
15. Using proper nomenclature for both genes (w and vg), specify the genotypes of: 4 marks
a. P1 generation . flies used in cross B
b. P1 generation o flies used in cross B
c. F1 generation ?s from cross B
d. F1 generation o's from cross B
16. Complete the Punnett Square below for Cross B. lo marks
F10 gametes
F19 gametes
Biology 207 Lab Manual

49E12E75-957B-49B9-A379-1E65D366104C.jpeg

WEEK 5: Page 14
Table 5-13: Distribution of critical values for the x2 distribution
df
0.50
0.30
0.20
0.10
0.05
0.02
0.01
0.005
UT A W NY
0.455
1.074
1.642
2.705
3.841
5.412
6.635
7.897
1.386
2.408
3.219
4.605
5.991
7.824
9.210
10.579
2.366
3.665
4.642
6.251
7.816
9.837
11.345
12.838
3.357
4.878
5.989
7.779
9.488
11.668
13.277
14.860
4.351
6.064
7.289
9.236
11.070
13.388
15.086
16.750
10
9.342
11.781
13.442
15.987
18.307
21.161
23.209
25.188
Search online for more extensive tables of critical values for the x2 distribution.

D3EE1676-C69A-488F-BDAF-3C3EAD5C0031.jpeg

in or xlinked.
WEEK 5: Page 12
Pattern of inheritance summary
11 marks
Using the symbols vg and vg for the mutant and wild-type alleles respectively, of a gene for wing shape,
complete the breeding diagram shown below for cross A and reciprocal cross B.
CROSS A
CROSS B (RECIPROCAL)
P1 generation
X
Phenotype
vestigial
wild-type
wild-type
vestigial
Genotypes
vg*/vg+
F1 generation
X
X
Phenotypes
Chom
wildtype
Genotypes
Expected Sex ratio
F2 generation
to
Phenotype(s)
Genotype(s)
Expected phenotypic ratio
3:1 wildtype to vestigial
Sex ratio
11 marks
Pattern of inheritance Summary
Using the symbols w and w for the mutant and wild-type alleles respectively, of a gene for eye colour, complete
the breeding diagram shown below for cross A and reciprocal cross B.
CROSS A
CROSS B (RECIPROCAL)
P1 generation
X
Phenotypes
white
red
red
white
Genotypes
w /Y
F1 generation
Phenotypes
red
Genotypes
Expected Sex Ratio
F2 generation
to
Phenotypes
Genotypes
w / w or w/w
Expected phenotypic ratio
Sex ratio
1:1 males to females
2019-2020

E886185B-4039-43DF-8FC9-07117680E27D.jpeg

WEEK 5: Page 10
7. Use the summary data for wing shape in Tables 5-7 and 5-8 for the observed distributions of F2 phenotypes in
10 marks
cross A and in cross B to perform x tests to substantiate a mode of inheritance for the gene for wing morphology.
a. Hypothesis (Ho):
b. Prediction:
Table 5-9: x tests with F2 generation phenotypes for wing morphology observed in crosses A and B.
OBSERVED
EXPECTED
CROSS
DISTRIBUTIONS (0)
DISTRIBUTIONS (E)
x' value
Critical
ACCEPT /
OF PHENOTYPES
OF PHENOTYPES
X-0.05
REJECT Ho
wild-type
vestigial | wild-type
vestigial
cross A F2
cross B F2
(reciprocal)
8. Can you accept or must you reject the hypothesis (H.) stated in 7a.? Explain. 3 morks
Analysis of the Drosophila white gene
Fill in the below tables using the compiled class data for the eye phenotype ONLY. 4 marks
Table 5-10: Summary of wing phenotypes in F1 and
Table 5-11: Summary of wing phenotypes in F1 and
F2 generations derived from cross A
F2 generations derived from cross B
Cross A Eye Colour
Females Males
Total
Cross B
Eye
Females Males
Total
P
(Reciprocal) Colour
A F1
Red
98
198
B F1
Red
100
197
95
292
White
3 3
30
63
White
65
29
qu
A F2
Red
94
189
BF
Red
70
90
White
29
32
61
White
32
32
5. What is the dominance relationship of the alleles that express the phenotypes of red eyes and white eyes,
respectively? Explain the basis for your answer. 3 marks
2019-2020

F41F1BEC-F9C6-4595-8BB4-AB4D281CA5DA.jpeg

+ = dominat
- = recessive
WEEK 5: Page 11
6. On the basis of the class data in Tables 5-10 and 5-11, circle the most likely pattern of inheritance of the gene
for eye colour. Substantiate your answer with specific observations from the data. 3 marks
Circle the most likely pattern:
X-linked
or
Autosomal
Substantiating observations:
9. Using class data in Tables 5-4 and 5-5 for the observed distributions of F2 phenotypes in cross A and in cross B,
perform x tests to substantiate a mode of inheritance for the gene for eye colour. 10 marks
a. Hypothesis (Ho):
b. Prediction:
Table 5-12: x2 tests with F2 generation phenotypes for eye colour observed in crosses A and B.
OBSERVED
EXPECTED
DISTRIBUTIONS (O)
DISTRIBUTIONS (E)
CROSS
OF PHENOTYPES
OF PHENOTYPES
x' value
Critical
ACCEPT /
red
white
red
white
X-0.05
REJECT Ho
O's
2s
O's
O's
cross A F2
cross B F2
(reciprocal)
10. Can you accept or must you reject the hypothesis (H.) stated in 9a.? Explain. 3 mork;
Biology 207 Lab Manual

F7EDCDA8-952F-45B6-9E38-87CD97EAA544.jpeg

E
R
F10
WEEK 5: Page 9
Analysis of Compiled Class Data Set
Use the chi square goodness of fit test to assess the F2 generations for both cross A and cross B (reciprocal) to
determine the likely dominance relationship and pattern of inheritance for the vestigial and white genes
independently.
The formula for the x2 Goodness of fit test is:
(0 -E)
Where:
E
x2 = the test statistic
[ = refers to the sum of all groups
O = the observed number of items within a group
shi
E = the expected number of items within a group according to the hypothesis
The chi square critical values table is located at the end of this lab section.
Analysis of the Drosophila vestigial gene
Fill in the below tables using the compiled class data for the wing phenotype ONLY. 4 marks
Table 5-7: Summary of wing phenotypes in F1 and
Table 5-8: Summary of wing phenotypes in F1 and
F2 generations derived from cross A.
F2 generations derived from cross B
Cross A Wing
Females | Males
Total
Cross B
Wing
Females
Males
Total
Shape
(Reciprocal) Shape
A F1
Normal
312
312
BF:
Normal
375
367 742
Vestigial
Vestigial
A F2
Normal
298
298
B F2
Normal
Vestigial
Vestigial
es .
X-linked
autos
or out
3. What is the likely dominance relationship of the alleles that express the phenotypes of wild-type wings and
vestigial wings, respectively? Explain the basis for your answer using the compiled class dataset. 3 marks
4. On the basis of the class data in Tables 5-6 and 5-7, circle the most likely pattern of inheritance of the gene for
wing morphology. Substantiate your answer with specific observations from the data.
3 marks
Circle the most likely pattern:
X-linked
or
Autosomal
Substantiating observations:
Biology 207 Lab Manual

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