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23/24. Shown below are the complentation test results involving 4 indepdendetly isolated lethal mutants in a

bacteriophage. Complentation was assayed by simultaneously infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complentated each other and therefore lysed open the bacteria, A "0" indicates no complentation and therefore no cell lysis occurred.

                                                      1     2      3    4

Test pair     Results              1    0    +      0    0

1,2                    +                   2           0     +     +

1,3                    0                   3                   0     0

1,4                    0                    4                          0

2,3                    +

2,4                     +

3,4                     0


23. Which mutants are in the same gene?

a)   1,3, & 4

b)   2,3 & 4

c)    1 &2

d)   2 &3

e)   1,2,3 &4


24. How many genes are there?

a)4 b)3  c)2 d)1  e)zero


25. DNA gyrase:

a)   Adds nucleoside to a DNA strand

b)   Cuts DNA

c)    Forms a covalent bond in a sugar phosphate backbone

d)   Adds supercoiling DNA

e)   Catalyzes hydrogen bonding


26. Enhancers:

a)   Are always located upstream from the TAT sequence

b)   Function in only one orientation

c)    Can be 2000 bp away from the TATA sequence

d)   All of the above


27. In eukaryotes, transcription factors:

a)   Bind directly to RNA polymerase NOT to the DNA

b)   Bind to the promoter before RNA polymerase binds

c)    Bind to the operator Not to the promotor

d)   Are NOT required for transcription


28. In eukaryotes, formation of mature mRNA BLANK RNA editing that is changing bases in the exon sequence

a)   Sometimes involves

b)   Never involves


29. Current human identification techniques can use:

a)STR polymorphisms located at several positions in the human genome

b)mitochondrial DNA

c)both of the above


30.. Histone acetyl transferase enzymes (HATS):

a     Acetylate histone proteins to increase transcription

b     Acetylate histone proteins to decrease transcription

c      Deacetylate histone proteins to increase transcription

d     None of the above


31. In the lactose operon, the "z" gene code for the enzyme galacrosidase, "o" is the operator gene and "I" is the repressor gene. If an e.coli strain with genotype I +o+z+/F'o^c is grown on medium which contains no lactose glucose, beta-galactosidase BLANK produced

a)is not b)is


32. cDNAs BLANK introns

a)do not contain b)contain 

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