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33. In eukaryotes, DNA replication:

a     Is catalyzed entirely by DNA polymerase


b     Starts from multiple origins on each chromosome

c      Does not require primer, only telomerase

d     Does not require any remodeling of chromatin

16. When a 21 kb fragment of DNA is cleaved with Hind3 the fragments formed are 12 and 9 kb. AluI cleavage produces fragments of 17 and 4 kb. A double digest with Hind3 and AIUI produces fragments of 9, 8 and 4 kb. Which of the following are true?

a)The AIUI site is in the 12 kb hind3 fragment

b)the AIUI site is in the 9kb Hind3 fragment

c)the Hind3 site is in the 4 kb AIUI fragment

d)AIUI cleaves at 2 sites within the 17 kb fragment

17. PCR uses:

a)   One incubation temperature over 90 C

b)   S1 nuclease

c)    Electrophoresis

d)   E.coli DNA piolymerase

e)   All of the above

18. In e.coli DNA replication is:



c)accompanied by synthesis in the 5 to 3 direction

d)started from several origins

e)all of the above are true

19. Telomeres:

a)   Can be active in immortal cells

b)   Contains RNA

c)    Adds bases to the ends of chromosomes

d)   All of the above

20.  A southern blot transfers BLANK to a nitrocellulose membrane

a)protein b)RNA c)DNA

21. In vertebrae genes, dispersed promoters:

a)start transcription at one specific base pair

b)are associated with genes that are transcribed continuously

c)are rare or absent

d)all of the above

40. Which of the following is false?

a)   Defective DNA repair enzymes can produce tumors

b)   Unregulated transcription factors can produce tumors

c)    Mutations in p53 prevent apoptosis

d)   A normal ras protein produces tumors 

15. Transgenic plants:

a)   Are cultivated in the Usa

b)can be made using Ti plasmid and plant tissue culture

c)may be resistant to insect pests

d)all of the above are true

16. The restriction endonucleases used in recombinant DNA work:

a)may produce sticky ends

b)recognize sequences 14-16 bp long

c)cut the DNA 15 bp away from the recognition sequence

d)all of the above are true 

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