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In the modern era of molecular genetics we now can place genes on plasmids and introduce them into bacteria. You

decide to introduce various genes from the Lac operon on to a multicopy plasmid call pB. Multicopy means that after the plasmid enters E. coli it multiplies and produces many copies of itself. Below are the phenotypes of various strains you have constructed. One of the strains below has a phenotype you would not expect.

Strain -IPTG +IPTG

  1. I + o+ Z+ Y+
  2. I + oc Z+ Y+
  3. pB o+ Z- Y- / I+ oc Z+ Y+
  4. pB I- o+ Z+ Y+ / I+ oc Z- Y- Blue Blue

a) Please fill in the above table with respect colony color.

b) Can you give an explanation for the colony colors of Strain 4.

c) Suggest two experiments to test your explanation for part "b".

Top Answer

a.While proceeding with a cloning experiment, colonies that have been transformed with the recombinant plasmid... View the full answer


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