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Question

You are a researcher performing work on recombinant vaccines in a proteomics lab. You are focusing on RSV

(Respiratory Syncytial Virus) and want to develop a subunit vaccine for it. You know a vaccine for this disease will be a boon for society, as it causes 160,000 infant mortalities annually. You believe the best vaccine candidate to be the surface glycoprotein G of RSV. You have spent the past few months carefully extracting the genome of RSV and are now ready to begin your work.





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You are a researcher performing work on recombinant vaccines in a proteomics lab. You are focusing on RSV
(Respiratory Syncytial Virus) and want to develop a subunit vaccine for it. You know a vaccine for this disease will
be a boon for society, as it causes 160,000 infant mortalityes annually. You believe the best vaccine candidate to be
the surface glycoprotein G of RSV. You have spent the past few months carefully extracting the genome of RSV and
are now ready to begin your work.
Q: After running a total of 27 cycles in the thermocycler, you get ready to run your results in an agarose gel.
According to GenBank, the total length of the genomic sequence for the RSV glycoprotein is 263 base-pairs. The
primers you designed select for all 263 base-pairs of this sequence. Where in the gel below do you expect to see
your product?
Ladder
PCR sample
10kbp
3kbp
1000bp
500bp
250bp

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