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help!!!! on the question that are bold Exercise #1 Watchthe online...

help!!!!

on the question that are bold


Exercise #1

Watch the online video tutorial "Culturing flies: Tutorial for exercise #1) and refer to the Drosophila lab manual by Carolina Publishing Company to ANSWER this exercise.


·       How long is the generation time of Drosophila melanogaster at 21°C? If we increase it to 25°C, will it elongate or shorten the generation time?

·       How many eggs can a single female fly lay in ten days?

·       How do we culture flies in labs? 

·      What is the purpose of adding the blue dye to the food?

·      How long did it take approximately for the food to solidify?

·      Take a picture of your prepared food and upload it here or in a separate file.

Exercise #2

Watch the online video tutorial "Anesthetizing flies: Tutorial for exercise #2) and refer to the Drosophilalab manual by Carolina Publishing Company to ANSWER this exercise.

Look in your Drosophila manual (Page 8-9) and find the answers to the following:

·       What are the four stages of the fruit fly life cycle?

·       How long does each stage take if flies are maintained at 21°C?

·       If a fly culture was set up a week prior to today and no flies have been removed, what life stages would you expect to find in the vial?

·       Use the images in the manual (Figure 4-7), can you find fruit flies at different stages of their lives? Write down the ones you can identify.

·       The flies we placed in the vial when setting up the culture are known to be the parental generation? Based on the life cycle of flies, when will their progenies become adults?

·       Now take a look at your fly vial labeled as wild type. When do you think the progenies will become adults?

·       Besides using a freezer and ice bath, what other methods can also be used to anesthetize flies for observations? Refer to your Drosophila lab manual.

·       What will happen if we over-anesthetize flies? For instance, if we leave the flies in the freezer too long?

Exercise #3: Identify fly sexes and mutants

3.1 For different sexes of fruit flies, refer to page 12 in your Drosophila manual or you can go to the online video tutorial "Identify fly sexes and mutations: Tutorial for exercise #3". Pause when you are done with Slide 3.


Describe the main differences between males and females?


Table 1: Summary of all mutant stocks used this semester. The fly cultures you obtained above are called stocks, which are a collection of flies with the same genotypes. Our flies in a stock are assumed to be true-breeding, pure, or homozygous for all their traits unless specified. Fly stocks of different phenotypes can be hybridized (or crossed) to yield hybrids also known as heterozygous flies. 


Image transcription text

Name of Phenotypes Single Gene Which Genotype flies Trait Trait Trait Trait mutan symbol chromosom #1: #2: Eye #3: #4: t? (abbreviatio e is the Eye shape Body Wing n of the fly gene on? color color shape stock name) Wild Dark Oval Grayish Straight N/A N/A ++ type red brown apterous vestigial ebony Sepia sepia.ebo ny white yellow Bar

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