A. Order the guide RNA targeting the coding region exon sequence of...

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111.0053? .197" First Order the guide RNA targeting the coding region exon sequence of ACE2 Order the guide RNA targeting the promoter of ACE2 Order the guide RNA targeting the termination sequence of ACE2 Inject the CRISPR CASQ protein and the selected guide RNA into a mouse embryo Inject the CRISPR CASQ protein and the selected guide RNA into all the lung cells of a young mouse Implant the embryo into a mother mouse and she will have baby mice. Place the embryos in a culture dish and allow the baby to develop. Do a PCR test on the young mouse that targets the ACE2 protein and sequence the PCR copies and make sure they have the desired gene sequence before going further. Infect the young mouse with a strain of COVID-19 that infects mice and compare to the non-edited control. (4 Pts) Select the four steps you would do in the correct order if you were going to test the work of Cao et al 2020 on gene edited mice. Then do step third is and fourth is

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