Asked by ahoyt3
I am needing help on #5. are my answers correct? . Comparative...
Image transcription text Comparative genetic analysis of the novel
coronavirus (2019-nCoV/SARS-CoV-2)
"Recent reports of the ACE2 expression analysis
receptor ACE2 in different populations
in lung tissues from Asian and Caucasian populations
are still controversial. The single-cell RNA-seq analysis
Yanan Cao , Lin Li, Zhimin Feng, Shengqing Wan, Peide Huang, Xiaohui Sun,
reported that the Asian donor had much higher ACE2
Fang Wen, Xuanlin Huang, Guang Ning & Weiqing Wang
expression cell ratio than white and African-American
Cell Discovery 6, Article number: 11 (2020) | Cite this article
donors "
5. (3 pts) Assume that the authors of this paper want to measure the ACE2 gene expression level
in humans representing different populations around the world.
Below are methods the geneticist could use in their research.
a. Run an agarose electrophoresis gel of the PCR product.
b. Conduct the RT-PCR in a real time thermal cycler such as a Taq Man
c. Obtain DNA sample from saliva cells from many people who have survived COVID-19
d. Obtain mRNA sample from cytoplasm of the lung lining cells of many people who have survived
COVID-19.
e.
Obtain mRNA sample from blood cells of many people who have survived COVID-19.
f. Run one of the coding region PCR tests on the sample shown in figure 1
g. Run an RT-PCR version of one of the coding region PCR tests shown in figure 1.
What are the three methods they should use, in the correct order, if they want to test the hypothesis
that there are differences in the levels of transcription of of the ACE2 protein among people.
First.
O . Then .And finally .
B
I am needing help on #5. are my answers correct?
Answered by p2014403
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