I am needing help on #8. 8. (4 pts). Assume that Cao et al (2020)...

Image transcription text

8. (4 pts). Assume that Cao et al (2020) from question 4 has the hypothesis that people who express the ACE2 protein at a higher level in their lung cells resist COVID-19 infections. You want to test this hypothesis on gene edited mice. Assume that the GeneScript company has designed guide RNAs that guide the Crispr Cas9 protein to make edits in different parts of the ACE2 gene. More than 20,000 In-Stock gRNA Constructs at just $99/clone Learn More GenScript Ace2 CRISPR guide RNA, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 CRISPR guide RNA[house mouse] Below are some genetics lab procedures... A. Order the guide RNA targeting the coding region exon sequence of ACE2 B. Order the guide RNA targeting the promoter of ACE2 C. Order the guide RNA targeting the termination sequence of ACE2 D. Inject the CRISPR CAS9 protein and the selected guide RNA into a mouse embryo E. Inject the CRISPR CAS9 protein and the selected guide RNA into all the lung cells of a young mouse F. Implant the embryo into a mother mouse and she will have baby mice. G. Place the embryos in a culture dish and allow the baby to develop. H. Do a PCR test on the young mouse that targets the ACE2 protein and sequence the PCR copies and make sure they have the desired gene sequence before going further. 1. Infect the young mouse with a strain of COVID-19 that infects mice and compare to the non-edited control. (4 Pts) Select the four steps you would do in the correct order if you were going to test the work of Cao et al 2020 on gene edited mice. third is and fourth is First Then do step Before you can continue with more steps. Bonus question on next page

... Show more

I am needing help on #8