Asked by ahoyt3
i am needing help on bonus question. that Cao . CE2 protein ~ test...
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. CE2 protein
Also assume that Tanaka et al (2020) discover point mutations that can change the way
COVID-19 binds to the ACE2 protein and reduces infection.
le Version 1 Preserved in Portico This version is not peer-reviewed
A Crispr-Cas9 System Designed to Introduce Point Mutations into the
Human ACE2 Gene to Weaken the Interaction of the ACE2 Receptor with
the SARS-CoV-2 S Protein
Pedro Tanaka, Jadson Santos, Ernna Oliveira , @Natane Miglioli , @Amanda Assis , Ana Monteleone-Cassiano ,(
Vitor Ribeiro , Max Duarte , Mayara Machado , Romario Mascarenhas , Alex Souza , Leticia Brito , Leticia
Oliveira , Eduardo Donadi , @Geraldo Passos*
Version 1 : Received: 6 May 2020 / Approved: 7 May 2020 / Online: 7 May 2020 (15:26:06 CEST)
Below are some genetics lab procedures...
A. Order the guide RNA targeting the coding region exon sequence of ACE2
B. Order the guide RNA targeting the promoter of ACE2
C. Order the guide RNA targeting the termination sequence of ACE2
D. Inject the CRISPR CAS9 protein and the selected guide RNA into a mouse embryo
E. Inject the CRISPR CAS9 protein and the selected guide RNA into all the lung cells of a
F. Implant the embryo into a mother mouse and she will have baby mice.
G. Place the embryos in a culture dish and allow the baby to develop.
H. Do a PCR test on the young mouse that targets the ACE2 protein and sequence the PCR
copies and make sure they have the desired gene sequence before going further.
I. Infect the young mouse with a strain of COVID-19 that infects mice and compare to the
BONUS (4 pts)
Select the four steps you would do in the correct order if you were going to
repeat and test the work of Tanaka et al 2020 on gene edited mice.
Then do step
and fourth is
Before you can continue with more steps.
... Show more
i am needing help on bonus question