Immune Assay Assignment: 1) What is the percentage of CD3+CD4+ T cells in this sample? A 10' 0.86% 27.77% 10 CD4-PE 10 100- 53.74% 17.636 10 CD3-FITC...
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Immune Assay assignment. See attached documents please. Please help me with this assignment! I want to know how to

analyze the images and arrive to the correct answer.







Screen Shot 2019-11-02 at 5.38.37 PM.png

Screen Shot 2019-11-02 at 5.38.37 PM.png

Immune Assay Assignment:
1) What is the percentage of CD3+CD4+ T cells in this sample?
A 10'
0.86%
27.77%
10
CD4-PE
10
100-
53.74%
17.636
10
CD3-FITC
CD11 and F4/80 are markers for macrophages. Which letter identifies the population
that is CD11b positive and F4/80 negative?
F4/80 (clone BM8) PerCP/Cyanine5.5
A
B
103
D
105
CD11b FITC
2) For an ELISA, why do you add a secondary antibody?
3) For a ELISA, why is there a blocking step?

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