READ THE INFORMATION AND ANSWER THE QUESTION BELOW. 1) Patient Number: 126 2) Missy - differential diagnosisUTI (urinary tract infection) Her...
This question has been answered
Question

READ THE INFORMATION AND ANSWER THE QUESTION BELOW. 1) Patient Number: 126

2) Missy - differential diagnosis UTI (urinary tract infection)

Her symptoms reveal that she has fever (a sign of infection).

She has difficulty in passing urine. Sometimes there is very little and at times no urine comes out even though she has the urge to pass urine. This is therefore the likely site of infection.

She feels bloated because her urine is getting retained in bladder and kidney's can no longer filter urine as there is a reflux of filtered urine back to the kidneys and all this together results in water retention and bloating.

3) Urine culture - this is the sample required to look for the infecting organism and prescribe needed antibiotic treatment from the antimicrobial susceptibility testing that will follow the culture results.

See culture 2:

culture 2.png

A. Name of the staining process or biochemical test

  • The staining process commonly used in identifying bacteria is Gram Staining. Biochemical tests are catalase tests, coagulase tests, IMVIC, MR/VP, citrate, urea hydrolysis test, PAD, carbohydrate fermentation tests.

B. What Exercise in your lab manual will you use to help you understand and interpret your results?

  • I think you will need the exercise wherein you observe the Gram stain and biochemical results and correlating it to the patient's clinical manifestations.

C. Give a brief explanation of what this test is looking for and how you will interpret the results. Be sure to answer these prompts in your response:

  • Gram Staining Result: Pink cocci: Gram negative cocci, Pink bacillus: Gram negative bacilli, Violet cocci: Gram Positive Cocci, Violet bacilli: Gram positive bacilli
  • IMVIC- Work up for enterobacteriaceae. H2S production will result to blackening of the medium, Red Ring Indole positive. Methy Red: Red color positive
  • Citrate: If the medium turns blue, the organism is citrate positive. If there is no color change, the organism is citrate negative. Some citrate negative organisms may grow weakly on the surface of the slant, but they will not produce a color change.
  • Hydrolysis of urea produces ammonia and CO2. The formation of ammonia alkalinizes the medium, and the pH shift is detected by the color change of phenol red from light orange at pH 6.8 to magenta (pink) at pH 8.1. Rapid urease-positive organisms turn the entire medium pink within 24 hours.
  • PAD: The phenylpyruvic acid produced by the action of phenylalanine deaminase produced by certain microorganism is detected by adding a few drops of 10% ferric chloride which result in the formation of a greencolored complex between these two compounds in the slant of the test tube indicating the positive test result
  • Carbohydrate Fermentation: The carbohydrate fermentation test is used to determine whether or not a bacteria can utilize a certain carbohydrate. Red: Negative ; Yellow: Positive

Your results are below:

Gram see stain - 14.

14.png

Catalase see biochem 46.

Screen Shot 2020-12-12 at 11.08.09 PM.png

Coagulase see biochem 50.

50.png

Urease see biochem 97.

97.jpg

1) Compile a report detailing your identification of the unknown bacterium. The report should contain the following sections:

Introduction: 

In the introduction section you would include patient information and unknown number, general information about the identification process and background information about the general type of disease (urinary tract infections, respiratory infections, gastrointestinal infections, etc).

Results Table: This table should include ALL staining and biochemical test results you requested (even tests that you requested but weren't that helpful in the identification process.) You do not need to explain the test or result, just a table showing the test results (e.g. + or - ). Some students like to include pictures, but this is completely optional.

Identification: 

Walk through your analysis during the identification process. Include staining, biochemical test results, research that you did, etc. This is where you include any difficulties in your analysis along with your justification for your identification. This section is where I see the reasoning and critical thinking you used in the identification process.

Conclusions:

In this section you will identify your unknown bacterial pathogen (written in correct binomial nomenclature) and give your final conclusions. You will also include a treatment plan for your patient and the prognosis. This is the place to wrap up the report with any final insights.

Citation List

You should include at least 3 references.

Answered by Expert Tutors

ctum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adip

m risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentes
Step-by-step explanation

m ipsum dolor

entesque dapibu

ec facilisis.


m ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar t


Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus



a. Fusce dui lect

ng elit. Nam lacinia pulvinar tortor nec facilisis. Pel


Bios for epidermidis.JPG



, dictum vitaec

sus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat,


m ring elit. Nam laciniinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentes


at, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat,m risus ante, dapibus a molestie coacinia pulvinar tortor nec facilisis


or nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lecet, consectetulsum dofficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laodictum vitae odio. Donec aliquet.iscing elit. Nam


ur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sitconsectetur aacinionec aliquet.u


Fusce dui lectus, congue vel laoreet ac, dictum vitae odaciniaDonec aliquet. Lorem ipsum dolor sit amet, consconsectetur aonec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus aec aliquet. Lorem ipsumicitur laoreet. Nam risus ante, dapibus a molestie consequat,


ur laoreet. Nam risuslestie consequat, ulto. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor n


itur laoree

tesque dapibus efficitur laoreet.officitur laoreet. Nam risuxFusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lect


fficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectusec aliquet. Lorem ipsum dolors a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet ac,ac, dictum vipsum dolor sit amet, cacinia pulvinar tortor nec facilisis.Donec aliquet. Lorem ipsum dolor sit amet, consectetur


ur laoreet

ac, dictum vit

sque dapibus efficitur laoreet. Nam risus ante, dapibus

ec aliquet. Lorem ipsum dolo

congue vel laoreet ac, dictum vitae odi

1 Attachment
Bios for epidermidis.JPG
JPG
The student who asked this found it Helpful
Overall rating 100%
Get unstuck

435,535 students got unstuck by Course
Hero in the last week

step by step solutions

Our Expert Tutors provide step by step solutions to help you excel in your courses