9B. · 50 mM Tris-HCl buffer, pH 7.6· 150 mM NaCl

Question

9B. · 50 mM Tris-HCl buffer, pH 7.6· 150 mM NaCl

Antibody level can be assessed by binding to an immobilised antigen. This would be done in a buffer such as Tris-buffered saline, with some detergent.

The components of this solution are:

· 0.05% (v/v) Tween-20

In the lab you have the following components:

· 2 M Tris-HCl buffer, pH7.6

· NaCl (solid, molecular weight 58.44 g/mol)

· Tween-20 (viscous liquid, molecular weight 1,227.54 g/mol)

How do you make up 500 ml of the solution?

9C. You have a control antibody in this solution, at a concentration of 5 µg/ml. You need to dilute it further in the same buffer to make 20 ng/ml, and you would like enough to add 100 µl to 10 wells. How would you make the dilution? (1 mark)

Answer 1

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