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1.a. What was the probable "etiological" agent (pathogen)?

 The Biol 2130 students and employees of a public school system developed diarrheal illness on April 2. The cafeteria served chicken that day. On April 1, part of the chicken was placed in water-filled pans and cooked in an oven for 2 hrs. at a temp of 177oC. The oven was turned off and the chicken was left overnight in the warm oven. Symptoms of the disease included nausea, vomiting, cramping and a fever. All the patients recovered within 24 hrs. Two "serotypes"of a Gram positive, oxidase negative, coagulase positive, cocci were isolated from 32 patients. 

b. What is the most likely source of his infection, and how would you verify this?


c. Explain the difference in an "intoxication" and an infection.


d. Determine if the illness was due to an intoxication or an infection and give the information you based your answer on.


e. What was the probable treatment protocol for this illness? (how were they treated)


f. Explain how this outbreak could have been prevented.



2. .The surgical ward at a public hospital was having an outbreak of diarrheal illness. The patients with the illness had all had surgery and were taking antibiotics as preventative measure to prevent infection.  Symptoms of the disease cramping, numerous bloody stools and inflammation of the colon. A Gram positive, endospore forming bacillus was isolated from 25 patients. Three of these patients died.


a. What was the probable "etiological" agent (pathogen)?


b. What is the most likely source of his infection, and how would you verify this?


c. Determine if the illness was due to an intoxication or an infection and give the information you based your answer on.


d. What was the probable treatment protocol for this illness? (how were they treated


e. Explain how this outbreak could have been prevented.

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