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-Why is it important to dilute your ENTIRE extract layer to a known

volume in a VOLUMETRIC FLASK before doing additional dilutions?
-You want to work this sample calculation. Smarty-pants Alexandra takes 10 mL of a solution that contains 0.2 mM methylene blue, as well as benzil and quinizarin. After separating the compounds using her extraction procedure, she dilutes her entire combined methylene blue layer to 25 mL in a volumetric flask. She then removes 5 mL of this solution and dilutes it to the mark in a 20 mL volumetric flask. She measures the absorbance as 0.506 in a 1 cm cuvette. What is her percent recovery of methylene blue?

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-Why is it important to dilute your ENTIRE extract layer to a known volume in a VOLUMETRIC FLASK before doing additional dilutions?
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