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How are the liquid and solid phases named in chromatography ? 1.2)

In TLC, when a polar solid support is used, which molecule will move up faster, polar or non-polar molecules ? Explain your answer 1.3) In the liquid extraction performed in the experiment, how is the aqueous layer removed ? 1.4) How is the petroleum ether solution dried in the experiment ? 1.5) Why cant you use ink to make a mark in a TLC plate ? 1.6) How much mobile phase should you add in the developing chamber ? Why is this relevant ? 1.7) When should you stop the TLC experiment after you introduce the plate in the chamber ? 1.8) Define retention factor. 1.9) Draw the resulting TLC plate if you spot on it fluorene and 9-fluorenone and develop the plate in ethyl acetate. 1.10) When is column chromatography used ? 1.11) Give an example of one of the most commonly used stationary phases in column chromatography. 1.12) Sketch a column chromatography system setup and ready to be used in a purification. Name each layer in the column. 1.13) Which mobile phases were used in the column chromatography shown in the video ? In what order ? Explain why. 1.14) Which of the components elutes first in the purification ? Explain why.

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