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Following electroporation of the pLL3-HRP plasmid into P . pastoris you test zeocin resistant colonies for the presence of HRP using a colony PCR...
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31.

Following electroporation of the pLL3-HRP plasmid into P. pastoris you test zeocin resistant colonies for the presence of HRP using a colony PCR assay as well as an HRP plate assay. If

you got a positive result for the colony PCR assay but a negative result for the HRP plate assay, what might explain your results?a.You forgot to add methanolb.You forgot to add sorbitolc.You forgot to add TMBd.You forgot to add Dextrosee.Both A and C could explain the results
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